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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator; the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service (tech@biolegend.com) for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
Product description and procedure summary:
The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Note: This procedure is optimized using 1mL whole blood per tube. Scale volumes in this procedure accordingly, e.g. using 2 mL whole blood, use twice the amount of nanobeads. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes.
Table 1. MojoSort™ Whole Blood Human CD8 Nanobeads Protocol: Separation Volumes and Magnet Times
Catalog No. | MojoSort™ Buffer Volume | Magnetic Incubation Time | |
---|---|---|---|
MojoSort™ magnet | 480019 | For samples ≤ 1 mL: Add 3mL buffer for separation | 5 minutes |
MojoSort™ magnet 14mL | 480020 | For samples ranging 1 – 5 mL: Top volume up to 10mL for each separation (e.g. for 5mL sample add 5mL buffer for the first separation, then add 10mL buffer for subsequent separations) | 10 minutes |
Application notes: To use this product in magnetic separation columns, a titration of the Biotin-Antibody Cocktail and Nanobeads should be performed. Optimal concentration for magnetic separation columns is lot-specific. Please contact BioLegend Technical Service (tech@biolegend.com) for further assistance on how to use MojoSort™ Nanobeads in magnetic separation columns.